Reporter

Part:BBa_J31012:Design

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-11-10)


Tet resistance expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 296
    Illegal NheI site found at 508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
    Illegal BamHI site found at 654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 680
    Illegal NgoMIV site found at 1048
    Illegal NgoMIV site found at 1208
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HixC-pBad-HixC-RBS-TetF-HixC BBa_J44012 was cut with XbaI/ PstI and inserted to the right of double terminator BBa_B0015 (SpeI/ PstI).


Source

Double terminator BBa_B0015 and HixC-pBad-HixC-RBS-TetF-HixC BBa_J44012

References